好氧厌氧潜流湿地微生物多样性与净化能力的关系赵艳

3111201111ActaScientiaeCircumstantiaeVol．31No．11Nov．2011No．308016No．2009ZX07010-0082009ZX07010-009SupportedbytheMinistryofEducationScientificResearchProjectNo．308016andtheNationalMajorScienceandTechnologyProgramforWaterPollutionControlandTreatmentNo．2009ZX07010-0082009ZX07010-0091987—[e-mail]*[e-mail]1987—[e-mail]*[e-mail]．2011．/J．31112423-2431ZhaoYLiFMWangHYetal．2011．Therelationshipbetweenmicrobialdiversityandwaterpurificationcapacityinaerobic/anaerobicsubsurfaceflowconstructedwetlandJ．ActaScientiaeCircumstantiae31112423-2431/11*21111．2661002．3004572011-01-172011-02-222011-03-10/CODNH+4-N．FDA、46-—2-DAPIBIOLOG、．OAO--OAOOBAAO----OBAAO．96hAWCDp＜0．01．COD、p＜0．01Simpson、Shannonp＜0．01SimpsonCODp＜0．05．．BIOLOGSimpson0253-2468201111-2423-09X171ATherelationshipbetweenmicrobialdiversityandwaterpurificationcapacityinaerobic/anaerobicsubsurfaceflowconstructedwetlandZHAOYan1LIFengmin1*WANGHaoyun2LIYang1ZHANGMingquan1WANGZhenyu11．CollegeofEnvironmentalScienceandEngineeringOceanUniversityofChinaQingdao2661002．TechnologyCenterofChemicalMineMetalMaterialTianjinEntry-ExitInspectionandQuarantineBureanTianjin300457Received17January2011receivedinrevisedform22February2011accepted10March2011AbstractAerobic/anaerobicsubsurfaceflowconstructedwetlandhadhighpurificationefficiencyforCODandNH+4-N．InordertoinvestigatethemechanismofbetterefficiencyofthistypeofwetlandFDADAPIandBIOLOGmethodswereusedtomeasuretheactivityquantityandfunctiondiversityofthemicroorganismsandtoanalyzetheirrelevancewithcapacityofwaterpurification．TheresultsindicatedthatalongwiththewaterflowtheactivityandquantityofmicroorganismsfirstdecreasedandthenincreasedinthegroupofOAOaerationwhiledecreasedallthewayinthegroupofOAOmultiplespotinfluentandOBAAOaeration．TheactivityfirstdecreasedandthenincreasedandthequantitygraduallydecreasedandincreasedatendinthegroupofOBAAOmultiplespotinfluent．Therelationshipbetweenthevalueof96hAWCDandtheirrespectiveoutflowtotalnitrogenremovalratewasextremelysignificantp＜0．01．ThequantityofmicroorganismswasrelatedtoCODremovalamounttotalnitrogenremovalrateandtotalnitrogensignificantp＜0．01andtheSimpsonandShannonindiceswerealsoextremelysignificantlyrelatedtototalnitrogenp＜0．01．TheSimpsonindicesandCODamountshadsignificantcorrelationp＜0．05．Theresultsprovidedbasicdataforrevealingtheeffectofdissolvedoxygenconditionontherelationshipbetweenmicrobialdiversityandpollutantremovalrateandprovidedevidenceforoptimizingtherunningofthesubsurfaceconstructedwetland．KeywordsconstructedwetlandmicrobialactivityandquantityBIOLOGfunctiondiversitytheindexofSimpsonpurificationcapacityDOI:10.13671/j.hjkxxb.2011.11.021311Introduction、、Hammer1989．Knight1950、、、、、、、Knight2000．Alexandros2009COD52.5%62．0%．2007COD85%．、、、、Kadlec1996．2010．2009、、2005BOD5TN．2010、/NH+4-N．CODCOD/N．OBAAO----COD、NH+4-N、TN91．6%、100%87．7%、TN2010．、．2Materialsandmethods2．120101．4①--OAO②--OAO③----OBAAO④----OBAAO．②④．①．1Fig．1Planofthenewconstructedwetland300d3～8cm1#、2#、3#、4#、5#1．4℃．2．22．2．1424211/FDAJohan1982Green20063g50mLpH=7．01mLFDA30℃3h2mL4℃、8000r·min－13min3mL490nmμg·g－1·h－1．33．2．2．2DAPIPorter19805g95mL0．85%200r·min－11h10mL0．2mLDAPI0．22μm．10Mcells·L－1．2．2．3BIOLOGEcoPlateTM8×1296331A2～H4A1．2009．5g45mL0．85%200r·min－11h10－2、10－3．10－3150μL·－11330℃7d24hBIOLOG590nm．2．3Excel2007SPSS18．0ANOVASpearman．BIOLOGAverageWellColorDevelopmentAWCDAWCD=C－()R/31CR．AWCDZak1994BIOLOG．1Magtlrran1988．1Table1FormulasformetabolicdiversitycalculationsofmicrobialcommunitiesShannonH'=－∑pilnpipiiC－RShannonShannonE=H'/lnSSSimpsonD=∑nini－1NN－1niiC－RNSimpson1/DMcIntoshEuclidianU=∑ni槡2niiC－RMcIntoshMcIntoshE=N－U槡N－N/SNS3Resultsanddiscussion3．1FDADAPI2．2a4OAO21．44μg·g－1·h－19．70μg·g－1·h－118．05μg·g－1·h－1524231OAOOBAAO34．91μg·g－1·h－110．16μg·g－1·h－167．21μg·g－1·h－18．32μg·g－1·h－117．93μg·g－1·h－1OBAAO．42b．OAOM107．987．167．68OAOOBAAOM8．077．817．716．967．59OBAAO．2Fig．2VariationofmicrobialactivityandamountamongfourdifferentwetlandtypesalongthecurrentsOAO2．23mg·L－11．07mg·L－1DO＜1～2mg·L－120073．OAOp＜0．05．OBAAO．NO－3-N．OBAAONH+4-N67．21μg·g－1·h－1．NO－3-N、NO－2-N4．0mg·L－1、0．37mg·L－1．．p＜0.050.33mg·L－1．624211/OBAAO3p＜0.01OBAAO．OAOOBAAOp＜0．05．OBAAOOBAAOp＜0．01OAO．OBAAONO－3-N、NO－2-NTN2010．OAOOBAAOOBAAOOBAAONO－3-N、NO－2-N、TNCOD．3．2BIOLOGECOTMAWCD3．3OAOAWCDOAOAWCD1#＞2#＞3#OBAAOAWCD1#＞2#＞4#＞5#＞3#OBAAOAWCD3#＞2#＞1#＞5#＞4#．OBAAOOBAAOAWCD41-2#、2-2#、3-3#、4-3#96hAWCD4．3Fig．3Variationofmicrobialmetabolicactivityamongfourdifferentwetlandtypes7242314AWCDFig．4AssociativerelationshipbetweenAWCDandoutflowTNremovalrateintheanaerobicsamplingssites96hAWCD4-3#＞2-2#＞1-2#＞3-3#44-3#＞2-2#＞1-2#＞3-3#p＜0．01OBAAO．96h55、6．5ShannonOAO3#2OBAAO3#4OBAAO4#、5#3．McIntoshOAO3#2OBAAO1#、3#、5#2#、3#3#、4#OBAAO1#、3#、4#2#、4#3#、5#．50．05Fig．5Comparisonoffourkindsofdiversityindexesamongdifferentsamplingsitesthesamelettermeansnosignificantdifferenceat0．05level6SimpsonOAO1#2OAO3#2OBAAO1#、4#3#4OBAAO4#、5#3．SimpsonOAO、824211/6Simpson0．05Fig．6ComparisonofSimpsondiversityindexofdifferentsamplingsitesthesamelettermeansnosignifican