包埋氨氧化细菌处理合成氨工业废水李辉军

89环境工程学报Vol．8No．920149ChineseJournalofEnvironmentalEngineeringSep．20141121*1．2100942．211500AOB、DO、FA、。25～30℃DO4．0～5．5mg/LFA＞6．5mg/L7．5%。X703A1673-9108201409-3791-06AmmoniaindustrialwastewatertreatmentusingimmobilizedammoniumoxidizingbacteriaLiHuijun1WuYajie1DaiXin2AnLichao11．SchoolofEnvironmentalandBiologicalEngineeringNanjingUniversityofScienceandTechnologyNanjing210094China2．NanjingKeshengEnvironmentalProtectionTechnologyCo．Ltd．Nanjing211500ChinaAbstractInthisstudytheammoniaoxidizingbacteriaAOBwereimmobilizedbypolyvinylalcoholandsodiumalginate．TheeffectsoftemperatureDOfreeammoniaFAorganicmatteronthestabilityofpartialni-trificationwereinvestigated．Theresultsshowedthatammoniaoxidizingbacteriapredominatedinthecarriersat25～30℃DOshouldbecontrolledat4．0～5．5mg/Litnotonlymettheneedsofbacterialgrowthbutalsoa-voidedexcessiveaeration．Otherwiseitwouldincreasethefrictionbetweenthecarriersresultinginthereductionoftheirsusefullife．Thecarrierswerestronglyresistanttonitrogenloadingrateandalsowithstoodthedamageoforganicmattertoammoniaoxidizingbacteria．TheactivityofAOBwasinhibitedwheninitialFAconcentrationwasover6．5mg/L．Afterenrichedammoniaoxidizingbacteriasludgehadbeenembeddedtheammoniaremovaleffi-ciencydecreasedabout7．5%．Inadditionanoxiczonewasformedinsidethecarrierswhileensuringpartialnitri-ficationprocess．Denitrificationcouldbeachievedintheanoxiczoneimprovingammoniaremovalefficiencyofthesystem．Keywordsimmobilizationpartialnitrificationammoniumoxidizingbacterianitriteaccumulationratedenitrification20120462013－08－092013－12－091988～。[e-mail]*[e-mail]20135800t130m3GB13458-201340mg/L17．4m37t201128．1t1/4。。NO－2→NO－3NOB25%、40%、50%、、2。AOB。NO－2NO－23。4-6。AOB、DO、8FA、-。11．1AOBAOBNH4Cl1．146g/L、NaH-CO32．5g/L、COD0．25g/L、K2HPO40．5g/L、FeSO4·7H2O0．010mg/L、MgSO4·7H2O0．30mg/L、CaCl2·2H2O0．18mg/LpH8．0。24hDO2mg/L20～30℃NAＲ90%AOB3．91×108/gMLSSMPN7。1．2100℃5．5%0．45%0．5%SiO230℃AOB90%1∶2．50．6%4℃24h30℃3．5h8。13～5cm。1750±50SiO2。1Fig．1ImmobilizedAOBcarriers1．32。1．5L8cm40cm。15%NaHCO3pH8．0、。12hNH+4-N、NO－2-N、NO－3-N。2Fig．2Schematicdiagramofexperimentaldevice1．49CODN-1--。NAＲ=NO－2-NNO－2-N+NO－3-N×100%1TN=NH+4-N+NO－2-N+NO－3-N2=TN－TNTN×100%322．115、20、25、3035℃。3NO－210mg/LAOB。AOBAOBNOB。35℃297393Fig．3Nitriteaccumulationatdifferenttemperatures。25℃、30℃15℃20℃AOBAOBAOBNOB10。325～30℃Yoo11、Balmelle12。3325、3035℃0．44、0．40、0．25kgNO－2-N/m3·dNAＲ78．0%、82．7%84．2%。25℃。25℃NAＲ35℃NOBNO－3-NAOB。2．2DODOAOBDOAOBDONOBNH+4→NO－213NOBNO－2。DO。4DO4、5、6、6．3mg/L25～30℃。DO5mg/LNO－2-NNAＲ209．43mg/L84．3%NH+4-N21．6mg/LDOAOBNOB。DO＞5mg/LNOBNO－2NO－2-NNOBDO。DO4．0～5．5mg/L。Yan14DODO4．2～4．6mg/LCODDO4．6mg/LCOD63．1%66．3%DO。4NO－x-NFig．4NO－x-Nconcentrationandnitriteaccumulationrateatdifferentdissolvedoxygenconcentrations2．3FApHAOBpH7．0～8．5。Anthonisen15FAAOBNOBAOB10～150mg/LNOB0．1～1．0mg/L。30±1℃10．69～320．79mg/LpH8．0DO4．0～5．5mg/LFA4FA1～30mg/L。FA=1714×NH+4-N×10pHe6334273+T+10pH45NAＲFAFA=6．5mg/LNAＲ97%FAFAAOBNOBNOBFA。FA=10～20mg/39738L99%NAＲFANO－3-NNOBNO－2。FANAＲ2FAAOBNO－2NOBNO－2NO－3。FANOBNAＲ。5NO－x-NFig．5NO－x-Naccumulationrateandammonianitrogenremovalefficiencyatdifferentfreeammoniaconcentrations2．4AOBDO16。6300mg/L12h。400mg/L93．0%200～300mg/L88．5%。AOB17。100、200、300400mg/LNAＲ83．3%、83．0%、81．8%83．8%。AOB。AOB。6Fig．6Ammonianitrogenremovalefficiencyandnitriteaccumulationrateatdifferentorganicmatterconcentrations2．5AOBAOBAOB2MLSS2500mg/L10h7。300mg/L2h43．66mg/L2h2h10h10h91．6%84．1%7．5%215．47189．21710hFig．7Treatmentefficiencyofsludgeandthecarriersin10hmg/LAOBAOB49739。2．619．7%3．5%。、。pHpH＜8．518。pH8．0。51955NH+4+76O2+109HCO－3→C5H7O2N+54NO－2+57H2O+104H2CO351/55≈1．8%3．5%17．9%。AOB。Bock20nitrosomonaseuropaea、nitrosomonaseutropha、。N2ON2。。399．9%80%。DO＞6．5mg/L。。。1．．20092891-5HanH．M．Advancementofchinaammoniaindustry．ChemicalIndustry20092891-5inChinese2．-．200016229-31YuanL．J．PengD．C．WangZ．Y．Short-cutnitrification-denitrificationbiologicalnitrogenremoval．ChinaWater＆Wastewater200016229-31inChinese3．．20064ChenH．H．LiuS．T．YangF．L．etal．Thedevelop-mentofsimultaneouspartialnitrificationANAMMOXanddenitrificationSNADprocessinasinglereactorfornitro-genremoval．BioresourceTechnology200910041548-15545OkabeS．OshikiM．TakahashiY．etal．Developmentoflong-termstablepartialnitrificationandsubsequentanam-moxprocess．BioresourceTechnology2011102136801-68076DavereyA．SuS．H．HuangY．T．etal．Partialnitrifica-tionandanammoxprocessAmethodforhighstrengthopto-electronicindustrialwastewatertreatment．WaterＲesearch20134792929-29377．．20088．．2007DaiX．Studyonmicrobialimmobilizationtechnologyforbi-ologicalnitrogenremoval．NanjingMaster’sDegreeThe-sisofNanjingUniversityofScienceandTechnology2007inChinese9．4．200210．．20084041152-1157ZhuG．B．PengY．Z．GuoJ．H．Biologicalnitrogenre-movalwithnitrificationanddenitrificationvianitritepath-way．JournalofHarbinInstituteofTechnology20084041152-1157inChinese11YooH．AhnK．H．LeeH．J．etal．Nitrogenremoval59738fromsyntheticwastewaterbysimultaneousnitrificationanddenitrificationSNDvianitriteinanintermittently-aera-tedreactor．WaterＲesearch1999331145-15412BalmelleB．NguyenK．M．CapdevilleB．etal．Studyoffactorscontrollingnitritebuild-upinbi