不同来源间充质干细胞生物学特性差异_方洪松

193220150806ChineseJournalofTissueEngineeringResearchAugust6,2015Vol.19,No.32ISSN2095-4344CN21-1581/RCODEN:ZLKHAH5243:B:2095-4344(2015)32-05243-062015-07-13:2015-07-13(430060)12CD90CD73CD105CD443,(81301592)19872015PubMed.[J].201519(32):5243-5248.doi:10.3969/j.issn.2095-4344.2015.32.029BiologicalcharacteristicsofdifferentsourcesofmesenchymalstemcellsFangHong-song,ZhouJian-lin,PengHao,DengShuang,WengJin-qing,LiuFeng,ChenSen,ZhouGuan-jin(DepartmentofBoneandJointSurgery,RenminHospitalofWuhanUniversity,Wuhan430060,HubeiProvince,China)AbstractBACKGROUND:Mesenchymalstemcells,underinvivoorinvitrospecificinductionconditions,candifferentiateintothecartilage,muscle,tendonsandsoon.Clinicaltrialsconcerningmesenchymalstemcellsmainlyincludetissuerepair(suchasbone,cartilageandjointrepair)andtreatmentofheart,liver,spinalcordinjuryandnervoussystemdiseases.OBJECTIVE:Tocomparethebiologicalcharacteristicsofmesenchymalstemcellsfromdifferentsources.METHODS:PubMedandCNKIdatabaseswereretrievedforarticlesrelatedtosourcesofmesenchymalstemcellsandbiologicalcharacteristicsofmesenchymalstemcellspublishedfrom1987to2015.Theretrievedarticlesweresummarizedandanalyzedinthefollowingaspects:cellsurfacemarker,proliferation,differentiation,migration,andfunction,soastoexplorethemeritsanddemeritsofmesenchymalstemcellsfromdifferentsources.RESULTSANDCONCLUSION:Adifferenceintheproliferationabilityandsurfacemarkersisfoundbetweendifferentsourcesofmesenchymalstemcells.Immunologicalcompetenceofmesenchymalstemcellsfromdifferentsourcesmaybecorrelatedwiththeiractivationstatus,speciesdifferences,tissuesourcesandculture.P.O.Box10002,Shenyang110180:MesenchymalStemCells;CellProliferation;CellDifferentiation;Antigens,SurfaceFunding:theNationalNaturalScienceFoundationofChina,No.81301592FangHS,ZhouJL,PengH,DengS,WengJQ,LiuF,ChenS,ZhouGJ.Biologicalcharacteristicsofdifferentsourcesofmesenchymalstemcells.ZhongguoZuzhiGongchengYanjiu.2015;19(32):5243-5248.0Introduction[1]1987Friedenstein[2]()[3-4]40[5-6][7-8]CD10CD13CD29CIM4CD90CD1CD14CD34CD45[9-10]CD105CD73CD44CD71CD90CD166(STRO-1)67811β[11]Tantrawatpan[12]Teotia[13]1Dataandmethods1.119872015PubMedmesenchymalstemcells;bonemarrow;umbilicalcord;placenta;amnioticfluid1.21.3521462Results2.1[14-15][16]CD90CD73CD105CD44CD45CD11bCD34[17]CD29CD44CD49eCD73CD90CD34CD45CD271.ISSN2095-4344CN21-1581/RCODEN:ZLKHAH5245[18]Lee[19]CD90CD166[20]SDCD11bCD29CD106CD90CD45CD49dSD36hPCROct-4Nanog[21][22]2CD29CD44CD34CD106[23]CD90CD73CD10590%22.2TBTTTTTBBG0/G1()IgAIgMIgG22HLA-1()α[2]10β1E22CD8+T10[24-25]BN[26]2.32G0G196.1%91.2%[27]10[28][29]1520d80%3813d80%810d80%357d80%Roura[30]3[31].P.O.Box10002,Shenyang110180[32-33][34-35][36-37][38-39][40][41]Li[42-44](1)3Clinicalapplicationandprospectofmesenchymalstemcells[45][46]1[44]3O33VonKossa3350µm()(VonKossa)(O).ISSN2095-4344CN21-1581/RCODEN:ZLKHAH5247[47]//CD166CD54CD102CD44CD106213467CαCD49aCD49bCD49cCD29CD104CD90CD105CD34CD45CD14CD3CD4CD8HLA-DR4References[1]LiedtkeS,FreytagEM,BoschJ,etal.Neonatalmesenchymal-likecellsadapttosurroundingcells.StemCellRes.2013;11(1):634-646.[2]FriedensteinAJ,ChailakhyanRK,GerasimovUV.Bonemarrowosteogenicstemcells:invitrocultivationandtransplantationindiffusionchambers.CellTissueKinet.1987;20(3):263-272.[3]IguraK,ZhangX,TakahashiK,etal.Isolationandcharacterizationofmesenchymalprogenitorcellsfromchorionicvilliofhumanplacenta.Cytotherapy.2004;6(6):543-553.[4]CampagnoliC,RobertsIA,KumarS,etal.Identificationofmesenchymalstem/progenitorcellsinhumanfirst-trimesterfetalblood,liver,andbonemarrow.Blood.2001;98(8):2396-2402.[5]LuLL,LiuYJ,YangSG,etal.Isolationandcharacterizationofhumanumbilicalcordmesenchymalstemcellswithhematopoiesis-supportivefunctionandotherpotentials.Haematologica.2006;91(8):1017-1026.[6]MaclaineSE,McNamaraLE,BennettAJ,etal.Developmentsinstemcells:implicationsforfuturejointreplacements.ProcInstMechEngH.2013;227(3):275-283.[7]WinterA,BreitS,ParschD,etal.Cartilage-likegeneexpressionindifferentiatedhumanstemcellspheroids:acomparisonofbonemarrow-derivedandadiposetissue-derivedstromalcells.ArthritisRheum.2003;48(2):418-429.[8]MizunoH,ZukPA,ZhuM,etal.Myogenicdifferentiationbyhumanprocessedlipoaspiratecells.PlastReconstrSurg.2002;109(1):199-209;discussion210-211.[9]FakhryM,HamadeE,BadranB,etal.Molecularmechanismsofmesenchymalstemcelldifferentiationtowardsosteoblasts.WorldJStemCells.2013;5(4):136-148.[10]Mohr