禽腺病毒家禽分离株的生物学特性

张明明,王小辉,秦爱建(,225009):(FAV),,3AFAV,,;,;SPF;1.01010TCID50,21SPF,,,,,FAV,,FAV:A;;中图分类号:S852.65+9.1文献标识码:A文章编号:05296005(2010)10000703BiologicalcharacteristicsoffowladenovirusstrainsisolatedfrompoultryZHANGMingming,WANGXiaohui,QINAijian(CollegeofVeterinaryMedicine,YangzhouUniversity,Yangzhou225009,China)Abstract:Tounderstandthepathogenicityofthreestrainsoffowladenovirus(FAV)subtypeAisolatedfrompoultry,somebiologicalcharacteristicswereexploredbyhemagglutinationtest,electronmicroscopy,cytopathictestandinoculationinchickenembryosorchickens.Theseisolateshadtypicalshapeofadenovirusandcouldnotagglutinatechickenredbloodcells.Theydidnotcausepathologicalchangesinchickembryofibroblasts,butthechickenembryokidneycellsbecameroundwithenhancedrefractivity.AndSPFchickenembryoswerealsonotaffected.Afterthe1dayoldSPFchickensweresubcutaneouslyororallychallengedwiththeisolateatthedoseof1.01010TCID50,noobviousclinicalsymptomsappeared.Andtheirbodyweightandimmuneorganweightwerenotaffected.Nolesionsappearedinmostorgansexcepttemporarychangesinliver.Therefore,theseFAVisolateshavelowpathogenicityandlargerbiosafety,andtheymaybebettercandidatesforFAVvector.Keywords:fowladenovirussubtypeA;biologicalcharacteristics;pathogenicityCorrespondingauthor:QINAijian:20100414:(2006AA10A205);(07KJA23021);(2005DKA212058):(1982),,,,Email:[e-mail]:,Email:[e-mail](fowladenovirus,FAV)(Adenoviridae),,FAV,[13](chickenembryolethalorphanvirus,CELOV)[4]retroviralsequencesinthechickengenomecloselyrelatedtoHPRS103(subgroupJ)avianleukosisvirus[J].GenVirol,1999,80:261268.[6]RuisBL,BensonSJ,ConklinKF.Genomestructureandexpressionoftheev/Jfamilyofavianendogenousviruses[J].Virol,1999,73:53455355.[7]SaccoM,HowesK,VenugopalK.IntactEAVHPendogenousretrovirusinSonneratsjunglefowl[J].Virol,2001,75:20292032.[8],.[M].2,:,1997:274275.[9],.pp38[J].,1994,10:239243.[10],,,.Jgp85[J].,2005,25(1):5459.[11]SambrookJ,FritschEF,ManiatisT.[M].,,.2:,1996:1922,4555,464467.7ChineseJournalofVeterinaryMedicine2010(46)10,PCRCELOVDNA,[5],3FAV,FAV11.1FAVFAVIC4YZ0605FAVID4YZ0605FAVIG24YZ0605,SPF,1SPF(CEF)(CEK)DMEMGibco1.2FAV1/10,,3000r/min5min,,,PEG6000,,1/10,,1.3CEKCEFDMEM1100FAV,0.2mL24CEKCEF,37!5%CO22h,,1%DMEM,5d,CEF,CEF,51.40.2mLFAV9SPF,37!5~6d,1.5FAVIG240605TCID50[6]1.01010TCID50,2101SPF,,,1020302,,,,22.1FAV,70~80nm,,1FAVIG2406051FAVIG24YZ0605A:13000;B:190002.2FAVCEKCEFFAVIC4YZ0605FAVID4YZ0605FAVIG24YZ0605CEF,;24h,CEK,,CEK,(2)2.3FAV,,,P1P2,3,2.4FAVSPF2.4.1FAV10,;2030,(1),10;2030,2.4.2FAVSPF,(3),10,,,,,,;20,82010(46)10ChineseJournalofVeterinaryMedicine1SPF(g)10203069.964.980.0128.3120.3121.5156.5151.2154.00.1400.1890.2550.5770.3630.5770.6850.7570.5600.0620.0450.0730.1510.1260.1300.1920.1560.2110.2760.2110.2870.7050.4080.5300.5480.5780.5523,FAV3,FAV,3FAV,IFAV,FAVCEKCEF,,,3FAVCEF,CEKFAV,,,,,∀FAV,#∃FAV∀FAV,3FAV,∀FAV,,1~2,3,,FAV,,,∀FAV1,10[7]21SPF,FAV,10,,2030,,FAVIG24YZ0605SPF,,,[8],10;,,,,FAVIG24YZ0605,,,FAV,,FAV1,3,,,,,1,3[9],参考文献:[1]RaueR,GerlachH,MllerH.Phylogeneticanalysisofthehexonloop1regionofanadenovirusfrompsittacinebirdssupportstheexistenceofanewpsittacineadenovirus(PsAdV)[J].ArchVirol,2005,150(10):19331943.[2]HollmnTE,FransonJC,FlintPL,etal.Anadenoviruslinkedtomortalityanddiseaseinlongtailedducks(Clangulahyemalis)inAlaska[J].AvianDis,2003,47(4):14341440.[3]GuyJS,BarnesHJ,SmithL,etal.Partialcharacterizationofanadenoviruslikevirusisolatedfrombroilerchickenswithtransmissibleviralproventriculitis[J].AvianDis,2005,49(3):344351.[4].1()[J].,2002,34(6):19.[5],,,.PCRDNA[J].,2003,18(2):137140.[6],.[M].2.:,1997:329331.[7]CookJ.Pathogenicityofavianadenovirusfordayoldchicks[J].JCompPathol,1974,84:505515.[8]SchonewilleE,SinghA,GbelTW,etal.Fowladenovirus(FAdV)serotype4causesdepletionofBandTcellsinlymphoidorgansinspecificpathogenfreechickensfollowingexperimentalinfection[J].VetImmunolImmunopathol,2008,121(12):130139.[9]YatesVJ,RheeYO,FryDE.Serologicalresponseofchickensexposedtoatype1avianadenovirusaloneorincombinationwiththeadenoassociatedvirus[J].AvianDis,1977,21(3):408414.9ChineseJournalofVeterinaryMedicine2010(46)10